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blasticidin resistance gene  (Addgene inc)


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    Structured Review

    Addgene inc blasticidin resistance gene
    Blasticidin Resistance Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blasticidin+resistance+gene+bsd/CLYBL-TO-hNGN2-BSD-mApple+(Plasmid+%23124229)/pm41578349-50-35-38
    Average 93 stars, based on 19 article reviews
    blasticidin resistance gene - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Construct:

    Article Title: miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis.
    Article Snippet: Annealed sgRNA oligonucleotides were ligated into BsmBI digested Lentiguide Puro (Addgene 52963) vector, resulting in the production of Lentiguide puro-scrambled sgRNA, -GSTK1 sgRNA1, and -GSTK1 sgRNA2. .. To produce a lentiviral construct expressing dCas9-VPR and a blasticidin resistance gene (BSD), dCas9-VPR (Addgene 63798) was subcloned first into the lentiviral construct having a CAG promoter; then P2A-BSD (Addgene 52962) was subcloned, resulting in the production of pBOB-CAG-dCas9-VPR-P2A-BSD. ..

    Article Title: miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis
    Article Snippet: Annealed sgRNA oligonucleotides were ligated into BsmBI digested Lentiguide Puro (Addgene 52963) vector, resulting in the production of Lentiguide puro-scrambled sgRNA, -GSTK1 sgRNA1, and -GSTK1 sgRNA2. .. To produce a lentiviral construct expressing dCas9-VPR and a blasticidin resistance gene (BSD), dCas9-VPR (Addgene 63798) was subcloned first into the lentiviral construct having a CAG promoter; then P2A-BSD (Addgene 52962) was subcloned, resulting in the production of pBOB-CAG-dCas9-VPR-P2A-BSD. .. For the luciferase assay, 3’ UTR of GSTK1 was amplified by PCR using cDNA from human neurons and ligated into a pGEM T easy vector (Promega A1360) first, then subcloned to a firefly luciferase construct expressed by a CAG promoter.

    Expressing:

    Article Title: miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis.
    Article Snippet: Annealed sgRNA oligonucleotides were ligated into BsmBI digested Lentiguide Puro (Addgene 52963) vector, resulting in the production of Lentiguide puro-scrambled sgRNA, -GSTK1 sgRNA1, and -GSTK1 sgRNA2. .. To produce a lentiviral construct expressing dCas9-VPR and a blasticidin resistance gene (BSD), dCas9-VPR (Addgene 63798) was subcloned first into the lentiviral construct having a CAG promoter; then P2A-BSD (Addgene 52962) was subcloned, resulting in the production of pBOB-CAG-dCas9-VPR-P2A-BSD. ..

    Article Title: miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis
    Article Snippet: Annealed sgRNA oligonucleotides were ligated into BsmBI digested Lentiguide Puro (Addgene 52963) vector, resulting in the production of Lentiguide puro-scrambled sgRNA, -GSTK1 sgRNA1, and -GSTK1 sgRNA2. .. To produce a lentiviral construct expressing dCas9-VPR and a blasticidin resistance gene (BSD), dCas9-VPR (Addgene 63798) was subcloned first into the lentiviral construct having a CAG promoter; then P2A-BSD (Addgene 52962) was subcloned, resulting in the production of pBOB-CAG-dCas9-VPR-P2A-BSD. .. For the luciferase assay, 3’ UTR of GSTK1 was amplified by PCR using cDNA from human neurons and ligated into a pGEM T easy vector (Promega A1360) first, then subcloned to a firefly luciferase construct expressed by a CAG promoter.



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